Analytical Data
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Gene name
ICAM-2/CD102
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简介
ICAM-2/CD102 protein belongs to the ICAM family of the immunoglobulin superfamily and may be involved in immune responses and cell interactions. This is consistent with the family's established role in immune processes. ICAM-2/CD102 Protein, Human (HEK293, His-Fc) is the recombinant human-derived ICAM-2/CD102 protein, expressed by HEK293 , with C-hFc, C-His labeled tag.
- Application
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Biological Activity
Determined by its ability to support the adhesion of PMA-stimulated jurkat cells to plates pre-coated with ICAM-2. The ED50 for this effect is 1.348 μg/mL, corresponding to a specific activity is 7.42×10^2 units/mg. Determined by its ability to support the adhesion of PMA-stimulated jurkat cells to plates pre-coated with ICAM-2. The ED50 for this effect is 1.348 μg/ml, corresponding to a specific activity is 7.42×102 units/mg.
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Alternative Names
Intercellular adhesion molecule 2; ICAM-2; CD102
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Species
Human
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Source
HEK293
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Tag
C-hFc;C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6FHE2
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Expression Region
K25-Q223
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AA Sequence
KVFEVHVRPKKLAVEPKGSLEVNCSTTCNQPEVGGLETSLDKILLDEQAQWKHYLVSNISHDTVLQCHFTCSGKQESMNSNVSVYQPPRQVILTLQPTLVAVGKSFTIECRVPTVEPLDSLTLFLFRGNETLHYETFGKAAPAPQEATATFNSTADREDGHRNFSCLAVLDLMSRGGNIFHKHSAPKMLEIYEPVSDSQ
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Protein Length
Partial
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Molecular Weight
85-95 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ICAM-2 (Intercellular Adhesion Molecule-2), also known as CD102, is an important cell adhesion molecule belonging to the immunoglobulin superfamily. It plays a critical role in the immune response by facilitating the adhesion of leukocytes to endothelial cells, which is essential for leukocyte trafficking during inflammation and immune surveillance. Researchers have shown that ICAM-2/CD102 is expressed in various tissues and is involved in processes like angiogenesis and the regulation of T cell activation. Given its significance in immune interactions and vascular biology, ICAM-2 has become a target of interest for therapeutic interventions in conditions such as autoimmune diseases, chronic inflammation, and cancer. The production of recombinant ICAM-2 proteins allows for in-depth studies of its structure-function relationship, interactions with ligands such as LFA-1 (Lymphocyte Function-associated Antigen 1), and its role in modulating immune responses. This research can unlock potential therapeutic strategies that exploit ICAM-2's adhesion properties, as well as enhance the understanding of immune mechanisms in health and disease. Thus, the study of ICAM-2/CD102 recombinant proteins is pivotal in elucidating the molecular pathways underpinning immune cell behavior and developing novel treatments for immune-related disorders.
