Analytical Data
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Gene name
HARS1
- Application
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Alternative Names
Histidyl-tRNA synthetase
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Species
Human
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Source
Baculovirus
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P12081
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Expression Region
2-504aa
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Molecular Weight
58.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HARS1, or Human Histidyl-tRNA Synthetase 1, is a crucial enzyme involved in protein synthesis, specifically in the attachment of histidine to its corresponding tRNA, a process vital for the proper translation of genetic information into functional proteins. Research into HARS1 has gained attention due to its emerging roles beyond housekeeping functions in protein synthesis, particularly in cellular signaling and potential involvement in various diseases. Aberrant expression of HARS1 has been linked to conditions such as cancer and autoimmune diseases, highlighting its significance in pathological contexts. Additionally, the enzyme's interactions with other biomolecules suggest it may play a role in regulating cell growth and differentiation. Recombinant HARS1 protein studies are essential for elucidating the enzyme's biochemical properties and understanding its physiological and pathological roles. Through techniques such as gene cloning, expression in suitable host cells, and purification, researchers aim to obtain functional HARS1 protein for structural and kinetic analysis. Overall, this research not only enhances our understanding of fundamental cellular processes but also opens avenues for potential therapeutic strategies targeting diseases associated with HARS1 dysregulation.
