Analytical Data
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Gene name
ALCAM/CD166
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简介
The ALCAM/CD166 protein is a cell adhesion molecule that mediates heterotypic and homotypic cell-to-cell contacts, promoting T cell activation, immune synapse formation, and LILRB4-mediated inhibition of T cell proliferation. ALCAM/CD166 Protein, Human (HEK293, His) is the recombinant human-derived ALCAM/CD166 protein, expressed by HEK293, with C-His labeled tag.
- Application
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Alternative Names
rHuCD166, C-Fc; ALCAM; CD-166
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Species
Human
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Source
HEK293
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13740
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Expression Region
W28-A526
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Protein Length
Extracellular Domain
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Molecular Weight
70-100 kDa,based on SDS-PAGE under reducing conditions,66-100 kDa,based on SDS-PAGE under non-reduced conditions,due to the glycosylation.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ALCAM (Activated Leukocyte Cell Adhesion Molecule), also known as CD166, is a member of the immunoglobulin superfamily and plays a crucial role in cell adhesion, migration, and immune response regulation. It is primarily expressed on activated immune cells and various tissues, and its interaction with integrins and other cell surface molecules is vital for leukocyte trafficking and tissue infiltration during inflammation and immune response. Studies have shown that ALCAM/CD166 is involved in tumor progression, metastasis, and the maintenance of cancer stem cells, making it a potential biomarker and therapeutic target for various malignancies. The recombinant protein of ALCAM/CD166 has been engineered to study its structure-function relationship and to elucidate its biological roles, providing insights into its mechanisms in cell signaling and adhesion processes. Research involving this recombinant protein aims to develop novel strategies for cancer immunotherapy and enhance our understanding of immune system dynamics. By investigating the interactions and pathways mediated by ALCAM/CD166, scientists hope to identify potential targets for drug development and improve treatment outcomes for patients with cancer and other immune-related disorders.
