Analytical Data
-
Gene name
S100B
-
简介
The S100B protein is a small zinc and calcium binding protein abundant in astrocytes with unique calcium and zinc binding sites of varying affinities. Although it binds weakly to calcium, it binds tightly to zinc, and potassium ions antagonize both. S100B Protein, Canine (HEK293, Fc) is the recombinant canine-derived S100B protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
-
Alternative Names
Protein S100; S100 calcium-binding; NEF; S100; 100beta; S100-B
-
Species
Canine
-
Source
HEK293
-
Tag
N-hFc
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
E2QTP3
-
Expression Region
S2-E92
-
Protein Length
Full Length of Mature Protein
-
Molecular Weight
40-45 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
S100B is a member of the S100 family of calcium-binding proteins, predominantly expressed in glial cells and to a lesser extent in neurons. It plays a crucial role in various cellular processes, including regulation of cell growth, differentiation, and apoptosis. Elevated levels of S100B in the bloodstream and cerebrospinal fluid have been associated with neurological disorders such as traumatic brain injury, Alzheimer’s disease, and stroke, making it a potential biomarker for these conditions. The research surrounding recombinant S100B protein focuses on its role in neuroinflammation and neuroprotection, where it can either promote or inhibit neuronal survival depending on the context. Scientists are particularly interested in its interactions with receptors like RAGE (Receptor for Advanced Glycation End Products) and its influence on downstream signaling pathways. Moreover, recombinant S100B serves as a valuable tool in studying its biochemical properties and therapeutic potential, aiding in the development of interventions for related neurodegenerative diseases. Understanding the dual roles of S100B and its downstream effects provides insights into the complex mechanisms of brain pathology, paving the way for novel strategies in diagnostics and treatment. In recent years, advances in protein expression techniques have facilitated the production of high-purity recombinant S100B, enabling more detailed investigations into its functional dynamics and its potential application in clinical settings. As research progresses, exploring the multifaceted roles of S100B may unveil new therapeutic avenues for managing various neurological conditions.
