Analytical Data
-
Gene name
NARS
-
简介
NARS proteins catalyze a two-step process that activates asparagine with ATP to form Asn-AMP and transfers it to the acceptor terminus of tRNA (Asn). NARS Protein, Human (sf9, His) is the recombinant human-derived NARS protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
-
Alternative Names
Asparagine--tRNA ligase, cytoplasmic; AsnRS; NARS1; NARS; NRS
-
Species
Human
-
Source
Baculovirus
-
Tag
N-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O43776
-
Expression Region
M1-P548
-
Protein Length
Full Length
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NARS (Asparaginyl-tRNA synthetase) is a crucial enzyme involved in the translation process of protein synthesis, specifically responsible for attaching asparagine to its corresponding tRNA. The study of NARS is particularly significant due to its implications in various biological processes and its potential involvement in diseases. Abnormalities in NARS function can lead to translational errors, contributing to conditions such as cancer and neurodegenerative disorders. Researchers have focused on characterizing the structure and function of NARS, exploring its role in aminoacylation and the broader implications for cellular metabolism and protein homeostasis. Advances in recombinant protein technology have enabled scientists to produce and analyze NARS in vitro, facilitating investigations into its enzymatic activity, inhibitor development, and structure-function relationships. Understanding the nuances of NARS will not only enhance our knowledge of translational regulation but also open avenues for therapeutic interventions targeting its dysfunction in pathological states. This line of research is integral to the broader understanding of protein synthesis and its critical role in health and disease, making NARS a focal point in molecular biology and therapeutic development.
