Recombinant Human EED Protein (Baculovirus)，His & GST

EED

EED protein is a core member of the Polycomb group (PcG) and is critical in the PRC2/EED-EZH2 complex, driving "Lys-9" and "Lys-27" methylation on histone H3 to achieve transcriptional repression . It recognizes trimethylated "Lys-26" on histone H1 and inhibits the methyltransferase activity of the PRC2 complex on nucleosomal histone H3 "Lys-27". EED Protein, Human (sf9, His-GST) is the recombinant human-derived EED protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

EED; HEED; Polycomb protein EED; WAIT1

Human

Baculovirus

N-His;N-GST

Greater than 90% as determined by SDS-PAGE.

O75530

M1-R441

Full Length

75-85 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.