Analytical Data
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Gene name
MFG-E8
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简介
MFG-E8 Protein plays a crucial role in maintaining intestinal epithelial homeostasis, facilitating mucosal healing, and contributing to VEGF-dependent neovascularization. It acts as a specific ligand, binding to alpha-v/beta-3 and alpha-v/beta-5 receptors, and participates in the phagocytic removal of apoptotic cells. The protein is also implicated in zona pellucida binding and is a major component of aortic medial amyloid. MFG-E8 Protein, Human (Biotinylated, sf9, His-Avi) is the recombinant human-derived MFG-E8 protein, expressed by Sf9 insect cells , with C-Avi, C-His labeled tag.
- Application
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Alternative Names
Lactadherin; HMFG; MFGM; MFG-E8; SED1
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Species
Human
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Source
Baculovirus
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Tag
C-Avi;C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q08431-1
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Expression Region
L24-C387
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Protein Length
Full Length of Isoform-1 Mature Protein
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MFG-E8, or Milk Fat Globule-EGF Factor 8, is a secretory glycoprotein that plays a crucial role in various physiological processes, including immune response, cell adhesion, and tissue repair. It is mainly expressed in various tissues, such as the lung, kidney, and brain, and is involved in the regulation of phagocytosis, angiogenesis, and inflammation. Research on recombinant MFG-E8 has gained significant attention due to its potential therapeutic applications, particularly in the context of inflammatory diseases and cancer. Studies have shown that MFG-E8 facilitates the clearance of apoptotic cells by macrophages, thereby promoting an anti-inflammatory milieu and attenuating autoimmune disorders. Furthermore, its role in tumor microenvironments has been investigated, as MFG-E8 is implicated in modulating macrophage polarization and enhancing tumor progression. The ability to produce recombinant MFG-E8 provides a valuable tool for elucidating its biological functions and exploring its utility in clinical settings. Thus, understanding the structure-function relationship of MFG-E8 through recombinant technologies may pave the way for novel therapeutic strategies targeting inflammatory diseases and cancer, highlighting the importance of this protein in both fundamental and applied biomedical research.
