Analytical Data
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Gene name
MSH2
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简介
MSH2 protein is a key component of the DNA mismatch repair system, which can recognize and bind DNA mismatches to initiate repair. MSH2 forms heterodimers MutS α and MutS β that target various mismatches. MSH2 Protein, Human (sf9, His-GST) is the recombinant human-derived MSH2 protein, expressed by Sf9 insect cells , with C-His, N-GST labeled tag.
- Application
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Alternative Names
DNA mismatch repair protein Msh2; hMSH2; MSH2
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Species
Human
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Source
Baculovirus
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Tag
C-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P43246-1
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Expression Region
M1-T934
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Protein Length
Full Length of Isoform-1
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MSH2 is a crucial protein involved in the DNA mismatch repair (MMR) system, playing a vital role in maintaining genomic stability by correcting base pair mismatches that occur during DNA replication. Mutations or malfunctions in the MSH2 gene can lead to microsatellite instability (MSI) and an increased risk of various cancers, especially colorectal cancer. Understanding MSH2 at the molecular level is essential for deciphering its function in DNA repair and its implications in tumorigenesis. Research on recombinant MSH2 proteins helps elucidate the protein's structure-function relationships and interactions with other components of the MMR pathway, including MSH3 and MLH1. By expressing and purifying MSH2 recombinant proteins, scientists can investigate their biochemical properties, such as conformational changes, binding affinity, and repair activity in vitro. This research not only enhances our understanding of MSH2's mechanism but also has potential clinical implications, paving the way for targeted therapies and diagnostic tools for MMR-deficient cancers. Consequently, ongoing studies on MSH2 recombinant proteins aim to provide insights that could contribute to cancer prevention and treatment strategies, making it a significant focus in cancer research and molecular biology.
