Analytical Data
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Gene name
DICER1
- Application
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Alternative Names
Dicer; HERNA; Dicer 1,Double-Stranded RNA-Specific Endoribonuclease; Helicase with RNase motif; Helicase MOI
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UPY3
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Expression Region
Leu6~Ser290
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Molecular Weight
33kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DICER1 is a crucial enzyme in the RNA interference (RNAi) pathway, responsible for processing precursor microRNAs (pre-miRNAs) into mature microRNAs (miRNAs) that regulate gene expression post-transcriptionally. Mutations in the DICER1 gene have been linked to various cancers, particularly in pediatric patients, establishing it as a significant player in tumorigenesis. The gene's role extends to the regulation of cellular processes such as development, differentiation, and the immune response, highlighting its biological importance. Researchers are increasingly interested in the study of DICER1 through the use of recombinant proteins, which allow for detailed investigations of its enzymatic functions, structure, and interactions with other cellular components. By producing DICER1 in recombinant form, scientists can dissect the mechanistic pathways underpinning its role in miRNA biogenesis and its implications in cancer biology. Furthermore, the study of DICER1 reaffirms the importance of small RNAs in gene regulation and opens avenues for potential therapeutic strategies targeting RNAi pathways in cancer and other diseases. Understanding the structure-function relationship of DICER1 through recombinant protein studies could pave the way for novel approaches in the modulation of gene expression, offering insights into both fundamental biology and clinical applications.
