Analytical Data
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Gene name
CD146/MCAM
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简介
The CD146/MCAM protein is a multifunctional adhesion regulator that plays a crucial role in maintaining endothelial monolayer adhesion in vascular tissue. Its expression is associated with promoting interactions between melanoma cells and vascular components, potentially enhancing hematogenous spread of tumors. CD146/MCAM Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse MCAM/CD146 is immobilized at 1 μg/mL, the concentration of Recombinant Mouse Galectin-3 that produces 50% of the optimal binding response is 0.4829 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Mouse MCAM/CD146 is immobilized at 1 μg/mL, the concentration of Recombinant Mouse Galectin-3 that produces 50% of the optimal binding response is 0.4829 μg/mL.
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Alternative Names
Cell surface glycoprotein MUC18; Gicerin; CD146; Muc18
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Species
Mouse
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Source
HEK293
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Tag
C-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q8R2Y2
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Expression Region
V24-V563
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AA Sequence
VPGEEKQPVPTPDLVEAEVGSTALLKCGPSRASGNFSQVDWFLIHKERQILIFRVHQGKGQREPGEYEHRLSLQDSVATLALSHVTPHDERMFLCKSKRPRLQDHYVELQVFKAPEEPTIQANVVGIHVDRQELREVATCVGRNGYPIPQVLWYKNSLPLQEEENRVHIQSSQIVESSGLYTLKSVLSARLVKEDKDAQFYCELSYRLPSGNHMKESKEVTVPVFYPAEKVWVEVEPVGLLKEGDHVTIRCLTDGNPQPHFTINKKDPSTGEMEEESTDENGLLSLEPAEKHHSGLYQCQSLDLETTITLSSDPLELLVNYVSDVQVNPTAPEVQEGESLTLTCEAESNQDLEFEWLRDKTGQLLGKGPVLQLNNVRREAGGRYLCMASVPRVPGLNRTQLVSVGIFGSPWMALKERKVWVQENAVLNLSCEASGHPQPTISWNVNGSATEWNPDPQTVVSTLNVLVTPELLETGAECTASNSLGSNTTTIVLKLVTLTTLIPDSSQTTGLSTLTVSPHTRANSTSTEKKLPQPESKGVV
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Protein Length
Extracellular Domain
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Molecular Weight
90-100 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CD146, also known as MCAM (Melanoma Cell Adhesion Molecule), is a cell adhesion molecule that plays a critical role in various physiological and pathological processes, including angiogenesis, immune response, and tumor metastasis. Identified initially in melanoma cells, CD146 is expressed in endothelial cells, smooth muscle cells, and a variety of other tissues, which suggests its involvement in both normal and diseased states. Research has demonstrated that CD146 is implicated in the regulation of cell-matrix interactions, influencing cell signaling pathways that govern proliferation and migration. Its role in cancer biology has garnered significant interest, as elevated CD146 expression has been associated with increased metastatic potential and poor prognosis in several tumor types. Consequently, the development of CD146/MCAM recombinant proteins has become a focal point for investigating its functions and interactions. These proteins serve as valuable tools for both in vitro and in vivo studies, allowing researchers to explore the molecular mechanisms underlying CD146-mediated cellular processes. Furthermore, therapeutic approaches targeting CD146 are being investigated, given its potential as a biomarker for cancer diagnosis and prognosis, as well as a target for novel cancer therapies. Understanding the precise roles and mechanisms of CD146/MCAM through the use of recombinant proteins is essential for advancing our knowledge of its contributions to human health and disease.
