Analytical Data
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基因名
ERP27
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简介
The ERP27 protein has a unique role in that it can specifically bind unfolded proteins and recruit the protein disulfide isomerase PDIA3 to the substrate through the hydrophobic pocket in its C-terminal domain. This interaction implies that ERP27 is involved in the unfolding stress response and resolves challenges in cellular proteostasis. ERP27 Protein, Human (HEK293, His) is the recombinant human-derived ERP27 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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别名
Endoplasmic Reticulum Resident Protein 27; ER Protein 27; ERp27; ERP27; C12orf46
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96DN0
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表达区间
E26-L273
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氨基酸序列
EVEKSSDGPGAAQEPTWLTDVPAAMEFIAATEVAVIGFFQDLEIPAVPILHSMVQKFPGVSFGISTDSEVLTHYNITGNTICLFRLVDNEQLNLEDEDIESIDATKLSRFIEINSLHMVTEYNPVTVIGLFNSVIQIHLLLIMNKASPEYEENMHRYQKAAKLFQGKILFILVDSGMKENGKVISFFKLKESQLPALAIYQTLDDEWDTLPTAEVSVEHVQNFCDGFLSGKLLKENRESEGKTPKVEL
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蛋白长度
Full Length of Mature Protein
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分子量
32-38 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The ERP27 protein, a member of the family of endoplasmic reticulum (ER) chaperones, plays a significant role in protein folding and quality control within the ER. Its importance has been underscored by research linking it to various cellular processes, including stress responses and the maintenance of proteostasis. ERP27 acts as a critical facilitator in the proper assembly of glycoproteins, thereby influencing the secretion efficiency of proteins and impacting overall cellular function. Furthermore, abnormalities in its expression and function have been associated with several diseases, including cancer and neurodegenerative disorders. Understanding the molecular mechanisms governing ERP27’s role in protein handling and its interaction with misfolded proteins is essential, as it may offer insights into therapeutic targets for disorders stemming from protein misfolding and accumulation. Recent studies have utilized advanced techniques to characterize ERP27's structure and functionality, emphasizing its potential as a biomarker for disease progression and response to treatments. The ongoing exploration of ERP27 not only sheds light on fundamental cellular mechanisms but also opens avenues for novel interventions in disease management through targeted modulation of its activity.
