Analytical Data
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基因名
BUB1
- Application
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别名
BUB1A; BUB1L; hBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O43683
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表达区间
Val787~Lys1085
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分子量
35kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
BUB1 (BUB1 Mitotic Checkpoint Serine/Threonine Kinase) is a crucial component of the spindle assembly checkpoint (SAC), a signaling pathway that ensures proper chromosome segregation during cell division. The protein is essential for monitoring the attachment of microtubules to kinetochores, preventing anaphase onset until all chromosomes are correctly aligned. Dysregulation of BUB1 has been implicated in various cancers, leading to aneuploidy and tumorigenesis due to its role in maintaining genomic stability. Research into the recombinant expression of BUB1 protein has emerged as a vital area of study, enabling detailed investigation of its structural and functional properties. By producing BUB1 as a recombinant protein, scientists can explore its kinase activity, interactions with other SAC components, and the mechanism underlying its role in cell cycle regulation. Such studies are critical for understanding the molecular basis of spindle checkpoint dysfunctions and may pave the way for developing targeted therapeutic strategies. Additionally, recombinant BUB1 protein serves as a valuable tool for high-throughput screening of potential inhibitors or modulators, facilitating the discovery of novel cancer treatments that could restore proper mitotic function in tumor cells.
