Analytical Data
-
Gene name
AFF1
- Application
-
Alternative Names
AF-4; AF4; AF4-MLL; MLL/AF4; MLLT2; PBM1; Myeloid/Lymphoid Or Mixed-Lineage Leukemia; Translocated To 2; Pre-B-Cell Monocytic Leukemia Partner 1
-
Species
Human
-
Source
E. coli
-
Tag
N-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P51825
-
Expression Region
Met1~Gly183
-
Molecular Weight
24kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
AFF1 (AF4/FMR2 family member 1) is a member of the AF4/FMR2 family of proteins, which are implicated in various cellular processes, including transcription regulation, RNA processing, and chromatin remodeling. Research into AFF1 has gained prominence due to its association with certain hematological malignancies, notably Acute Lymphoblastic Leukemia (ALL), where it is often found fused with other genes through chromosomal translocations. These aberrations can result in the production of fusion proteins that disrupt normal cellular functions and contribute to oncogenesis. Additionally, AFF1 plays a significant role in the development of neuronal tissues, linking it to neurodevelopmental disorders when functionally impaired. Investigating the structure and function of recombinant AFF1 protein is vital for understanding its biological roles and mechanisms of action. This research can facilitate the discovery of potential therapeutic targets within the pathways regulated by AFF1, offering hope for innovative treatments for related cancers and neurodevelopmental conditions. By characterizing AFF1’s interactions, post-translational modifications, and functional domains, scientists aim to elucidate its contribution to disease pathology and uncover novel insights into the regulatory networks it influences.
