Analytical Data
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Gene name
NDUFS1
- Application
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Alternative Names
CI-75Kd; NADH-Coenzyme Q Reductase; NADH Dehydrogenase Iron-Sulfur Protein 1; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P28331
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Expression Region
Ala524~Cys727
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Molecular Weight
26kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NDUFS1 is a critical subunit of the mitochondrial respiratory chain complex I, also known as NADH:ubiquinone oxidoreductase, which plays a vital role in cellular energy production through oxidative phosphorylation. Deficiencies or mutations in the NDUFS1 gene have been implicated in a range of mitochondrial disorders, resulting in severe clinical phenotypes, including neurodegenerative diseases and myopathies. Understanding the function and structure of NDUFS1 is essential for elucidating its role in mitochondrial bioenergetics and for developing potential therapeutic strategies to address associated pathologies. Recent advances in recombinant protein technology have enabled the expression and purification of NDUFS1, facilitating in-depth studies of its enzymatic activity, interaction with other mitochondrial components, and its impact on overall cellular metabolism. These studies not only contribute to our understanding of mitochondrial dysfunction but also pave the way for innovative treatments targeting the underlying causes of mitochondrial diseases. As a result, recombinant NDUFS1 protein research is crucial for both basic science and translational medicine, highlighting its significance in the field of bioenergetics and genetic disorders.
