Analytical Data
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Gene name
SDHA
- Application
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Alternative Names
SDH2; SDHF; FP; Flavoprotein; Flavoprotein subunit of complex II; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
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Expression Region
Val4~Ile300
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Molecular Weight
36kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Succinate dehydrogenase (SDH) is a critical enzyme complex involved in the mitochondrial respiratory chain and the tricarboxylic acid (TCA) cycle, playing a key role in cellular energy production and metabolism. Dysregulation of SDH is associated with various pathologies, including hereditary paraganglioma, pheochromocytoma, and specific types of cancer, making it a significant target for biomedical research. The study of recombinant SDHA (the flavoprotein subunit of SDH) is particularly important for understanding its structural and functional properties, as well as its interactions with other subunits and metabolites. Producing recombinant SDHA allows for detailed biochemical studies, facilitating the exploration of enzyme kinetics, substrate specificity, and response to inhibitors, thus enhancing our knowledge of its role in health and disease. Furthermore, investigating SDHA mutations can elucidate their impact on enzyme functionality, contributing to the development of therapeutic strategies for related disorders. As such, SDHA recombinant protein research not only aids in understanding fundamental biological processes but also holds potential for translational applications in disease treatment and prevention.
