Analytical Data
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Gene name
DKK-1 C terminal Domain
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简介
DKK-1 Proteinas are potent antagonists of canonical Wnt signaling, inhibiting LRP5/6-Wnt interactions and forming a ternary complex with KREMEN to promote LRP5/6 internalization.In addition to its proapoptotic function by antagonizing KREMEN1 independently of Wnt, DKK-1 also exhibits antiapoptotic activity.DKK-1 C terminal Domain Protein, Human (HEK293, Fc-Avi) is the recombinant human-derived DKK-1 C terminal Domain protein, expressed by HEK293 , with C-Avi, C-hFc labeled tag.
- Application
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Alternative Names
Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK
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Species
Human
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Source
HEK293
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Tag
C-Avi;C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O94907
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Expression Region
M178-H266
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Protein Length
Partial
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Molecular Weight
45-55 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Recombinant proteins of the C-terminal domain of DKK-1 (Dickkopf-related protein 1) have garnered significant interest in biomedical research due to their crucial role in modulating the Wnt signaling pathway, which is essential for various cellular processes including embryonic development, tissue homeostasis, and stem cell regulation. DKK-1 functions as an antagonist of the Wnt pathway, inhibiting the signaling to prevent aberrant cell proliferation and differentiation. Its dysregulation is implicated in numerous pathological conditions, such as cancer, osteoporosis, and neurodegenerative diseases. The study of the C-terminal domain of DKK-1, which is key to its binding with the receptor LRP5/6, allows researchers to better understand its mechanism of action and potential therapeutic implications. By producing recombinant DKK-1 C-terminal domain proteins, researchers can explore their structural properties, interaction dynamics, and biological effects in laboratory settings. This knowledge may lead to the development of novel strategies for manipulating Wnt signaling in disease contexts, offering prospects for targeted therapies aimed at restoring normal cellular functions or inhibiting tumorigenesis. Consequently, the investigation of recombinant DKK-1 C-terminal domain proteins is pivotal for advancing our understanding of cellular signaling networks and their relevance in health and disease.
