Analytical Data
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Gene name
ISG15 E1/UBE1L
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简介
The ISG15 E1/UBE1L protein initiates ubiquitination by adenylating the C-terminal glycine of ubiquitin with ATP to form a thioester bond with its cysteine residue. This produces ubiquitin-E1 thioester and free AMP. ISG15 E1/UBE1L Protein, Human (sf9, His, Strep) is the recombinant human-derived ISG15 E1/UBE1L protein, expressed by Sf9 insect cells , with N-8*His, N-Strep labeled tag.
- Application
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Alternative Names
Ubiquitin-like modifier-activating enzyme 7; UBA7; Ubiquitin-activating enzyme 7; D8; Ubiquitin-activating enzyme E1 homolog; UBE1L; UBE2
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Species
Human
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Source
Baculovirus
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Tag
N-StrepⅡ;N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P41226
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Expression Region
D2-L1012
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Protein Length
Partial
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Molecular Weight
114.7 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ISG15 (Interferon-stimulated gene 15) is a ubiquitin-like protein that plays a crucial role in the immune response, particularly in the regulation of antiviral and inflammatory processes. The E1 enzyme UBE1L (ubiquitin-activating enzyme 1-like) is responsible for the initial step in the ISG15 conjugation pathway, activating ISG15 for subsequent transfer to target proteins, a process known as ISGylation. This post-translational modification has been implicated in various biological functions, including protein stability, localization, and activity modulation, thereby influencing cellular responses to stress and pathogen invasion. Research has shown that ISGylation can enhance the antiviral capabilities of host cells and modulate the immune system's response, making it a key area of interest in the study of host-pathogen interactions and immune regulation. The exploration of ISG15 and UBE1L, particularly through recombinant protein studies, provides valuable insights into their functional mechanisms and their potential as therapeutic targets in diseases characterized by dysregulated immune responses, such as cancer and autoimmune disorders. Understanding the biochemistry and biological roles of both ISG15 and UBE1L can pave the way for novel strategies in the development of antiviral therapies and immunomodulatory treatments.
