Analytical Data
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Gene name
Midkine
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简介
Midkine (MDK) is a multifunctional cytokine and growth factor that signals through cell surface proteoglycan and non-proteoglycan receptors. It affects inflammatory responses, cell proliferation, adhesion, survival, tissue regeneration, differentiation and migration. Midkine Protein, Mouse is the recombinant mouse-derived Midkine protein, expressed by E. coli , with tag free.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Human Midkine Protein, premium grade at 2 μg/mL (100 μL/well) can bind Human LRP-6. The ED50 for this effect is ≤109.8 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human Midkine Protein, premium grade at 2 μg/mL (100 μL/well) can bind Human LRP-6, the ED50 for this effect is 109.8 ng/mL.
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Alternative Names
Midkine; Mdk; MK; Retanoic acid-responsive protein; Retinoic acid-induced differentiation factor
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Species
Mouse
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P12025
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Expression Region
K23-D140
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Protein Length
Full Length of Mature Protein
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Molecular Weight
15 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Midkine (MK) is a heparin-binding growth factor with a significant role in various physiological and pathological processes, including embryogenesis, neural development, and tissue repair. Its expression is induced in response to injury, inflammation, and cancer, suggesting its involvement in tumor progression and metastasis. Research has shown that Midkine participates in critical cellular functions, such as cell proliferation, migration, and differentiation. Due to its multifunctional nature, MK has garnered interest as a potential therapeutic target for various diseases, including cancer and neurodegenerative disorders. The recombinant protein form of Midkine is particularly valuable for research and clinical applications, providing a tool to study its biological functions and interactions in detail. Advances in recombinant DNA technology have enabled the production of functional Midkine proteins, facilitating investigation into their mechanisms of action and potential as a biomarker for disease progression. Moreover, the therapeutic applications of Midkine, such as in drug delivery systems and regenerative medicine, are under exploration, highlighting its potential in enhancing healing processes and improving outcomes in various medical conditions. As research progresses, further elucidation of the role of Midkine in health and disease may open new avenues for targeted therapies, emphasizing the importance of understanding this protein in both basic science and clinical contexts.
