Analytical Data
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基因名
Nicotinamide N-Methyltransferase/NNMT
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简介
The nicotinamide N-methyltransferase (NNMT) protein catalyzes the methylation of nicotinamide using S-adenosyl-L-methionine to form N1-methylnicotinamide. NNMT affects pluripotent embryonic stem cell development and acts as a metabolic regulator, affecting adipose tissue energy expenditure, gluconeogenesis, and cholesterol biosynthesis. Nicotinamide N-Methyltransferase/NNMT Protein, Human (His) is the recombinant human-derived Nicotinamide N-Methyltransferase/NNMT protein, expressed by E. coli , with C-His labeled tag.
- Application
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生物活性
1. Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 0.0488 μg/mL, corresponding to a specific activity is 2.049×104 units/mg. 2. Measured by its ability to methylate nicotinamide. The specific activity is 114.696 pmol/min/μg. Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 0.0488 μg/mL, corresponding to a specific activity is 2.049×104 units/mg.
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别名
Nicotinamide N-methyltransferase; NNMT
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种属
Human
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表达系统
E. coli
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P40261
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表达区间
M1-L264
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氨基酸序列
MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL
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蛋白长度
Full Length
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分子量
25-30
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Nicotinamide N-methyltransferase (NNMT) is an important enzyme involved in the metabolism of nicotinamide, which plays a crucial role in various biological processes, including energy metabolism and cellular signaling. Elevated levels of NNMT have been associated with several metabolic disorders, including obesity and type 2 diabetes, making it a potential therapeutic target. Additionally, NNMT has been implicated in cancer progression and drug metabolism due to its role in modulating the availability of nicotinamide coenzymes and methyl groups. The recombinant protein form of NNMT is especially significant for research, as it allows for detailed studies of its enzymatic properties, substrate specificity, and inhibitor design. Understanding NNMT's structure-function relationship can provide insight into its biological roles and facilitate the development of novel pharmacological strategies for metabolic diseases and cancer. Therefore, producing and characterizing recombinant NNMT is a critical step in elucidating its function and therapeutic potential. Researchers employ various techniques, including molecular cloning and expression systems, to obtain functional NNMT, which can then be used in biochemical assays to explore its activity and regulation in different physiological contexts. Overall, the study of recombinant NNMT is essential for advancing our understanding of its role in human health and disease.
