Analytical Data
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基因名
Toxin B/TcdB 蛋白
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简介
Toxin B/TcdB protein is the precursor of the cytotoxin TcdB, which can strategically destroy the colon epithelium and cause host inflammatory response and diarrhea in Clostridium difficile infection. Compared with TcdA, TcdB has higher virulence and is essential for eliciting host responses. Toxin B/TcdB Protein, C. difficile (His) is the recombinant Toxin B/TcdB protein, expressed by E. coli , with C-6*His labeled tag.
- Application
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别名
Toxin B; Glucosyltransferase TcdB; toxB
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种属
Others
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表达系统
E. coli
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P18177
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表达区间
M1-L543
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氨基酸序列
MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVEKYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVWIGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFRENLNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEIDELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISALKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEHFDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKDSYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMELGKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNISQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSL
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蛋白长度
Partial
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分子量
65 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Toxin B (TcdB) is one of the primary virulence factors produced by Clostridium difficile, a bacterium responsible for antibiotic-associated diarrhea and severe colitis. This large clostridial toxin is known for its ability to glucosylate Rho family GTPases, leading to cytoskeletal disruption and cell death, contributing to the pathogenesis of C. difficile infection (CDI). The rising incidence of CDI, particularly in healthcare settings and among antibiotic-treated patients, has become a significant public health concern. As traditional antibiotic therapies often fail to eliminate the infection and can lead to recurrences, there is an urgent need for novel therapeutic approaches. Recombinant TcdB has emerged as a promising candidate for vaccine development and immunotherapeutic strategies. Through the use of molecular cloning and expression systems, researchers have successfully produced recombinant TcdB, enabling detailed studies on its structure, function, and immunogenic properties. These studies are crucial for understanding the toxin's mechanisms and for developing vaccines that can elicit robust immune responses. Additionally, recombinant TcdB can serve as a valuable tool for research into CDI pathogenesis and for screening potential therapeutic agents. Overall, the study of TcdB and its recombinant forms is pivotal in addressing the challenges posed by C. difficile infections, fostering advancements in preventive and therapeutic measures that could significantly reduce the burden of this disease.
