Analytical Data
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Gene name
HNF1b
- Application
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Alternative Names
FJHN; TCF2; HNF1beta; HNF2; LFB3; LF-B3; MODY5; VHNF1; Transcription Factor 2,Hepatic; Variant Hepatic Nuclear Factor; Homeoprotein LFB3
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P35680
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Expression Region
Pro159~Phe300
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Molecular Weight
20kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
HNF1b (Hepatocyte Nuclear Factor 1 Beta) is a crucial transcription factor that plays a significant role in the regulation of various genes involved in kidney and liver development, pancreatic function, and glucose homeostasis. Mutations in the HNF1b gene have been linked to a range of congenital disorders, including renal cysts, maturity-onset diabetes of the young, and other developmental abnormalities. Given its importance in both developmental biology and metabolic diseases, HNF1b is a focal point of research aimed at understanding its structure, function, and interactions with other cellular components. The production of recombinant HNF1b protein has become essential for these studies, as it allows for in vitro functional assays, characterization of protein-DNA interactions, and investigation of its role in signaling pathways. Techniques such as molecular cloning, expression in suitable host systems, and purification protocols have been developed to generate high-quality recombinant HNF1b. Understanding the biochemical properties and functional domains of HNF1b through the study of its recombinant form not only helps in deciphering the molecular mechanisms underlying its various biological roles but also paves the way for potential therapeutic interventions targeting HNF1b-related disorders. Therefore, ongoing research into HNF1b is critical for advancing our knowledge of its multifaceted functions and its implications in human health and disease.
