Analytical Data
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Gene name
LEDGF
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简介
LEDGF p52 protein is involved in essential functions, such as DNA-binding and chromatin binding activity. It positively regulates transcription and plays a role in mRNA 5'-splice site recognition. The protein is located in heterochromatin, the nuclear periphery, and the nucleoplasm. It is ubiquitously expressed in various tissues, including the brain (RPKM 41.2), ovary (RPKM 33.4), and 24 other tissues. LEDGF p52 Protein, Human is the recombinant human-derived LEDGF p52 protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
PC4 And SFRS1 Interacting Protein 1; LEDGF; DFS70; PSIP2; P52; P75; Lens Epithelium-derived Growth Factor
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75475-2
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Expression Region
M1-Q333
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AA Sequence
MTRDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNNPKVKFSSQQAATKQSNASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDITTPKAARRGRKRKAEKQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQKEEDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGDDQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEEQMETEHQTTCNLQ
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Protein Length
Full Length of Isoform-2
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Molecular Weight
51 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
LEDGF (Lens Epithelium-Derived Growth Factor), also known as PSIP1, is an essential co-factor in the integration of viral DNA into the host genome, particularly in the context of HIV-1 infections. The study of LEDGF has gained prominence due to its role in promoting the integration of viral genomes, which is critical for viral replication and persistence in infected cells. As a transcriptional co-activator, LEDGF interacts with the HIV-1 integrase and the chromatin, facilitating the integration process. Furthermore, it has been implicated in various cellular processes beyond viral interactions, including cell survival, differentiation, and stress responses. Research into LEDGF’s structure and functions has potential therapeutic implications; targeting its activity could provide novel strategies for antiviral therapies, especially in the fight against HIV. Additionally, the LEDGF protein has garnered attention in cancer research, as its overexpression can influence tumor growth and progression. The recombinant production of LEDGF protein aids in understanding its biochemical properties and interactions at a molecular level, thus enhancing our insights into both viral pathogenesis and cellular regulation mechanisms. The ongoing exploration of LEDGF’s multifaceted roles continues to unveil its significance in virology and cell biology, making it an important focus for future research in therapeutic developments.
