Analytical Data
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Gene name
EAAT5
- Application
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Alternative Names
SLC1A7; AAAT; Solute Carrier Family 1 Member 7,Glutamate Transporter; Retinal glutamate transporter
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00341
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Expression Region
His115~Thr216
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Molecular Weight
15kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EAAT5 (excitatory amino acid transporter 5) is a member of the glutamate transporter family, predominantly expressed in the retina and certain regions of the brain. It plays a crucial role in clearing glutamate from the synaptic cleft, thereby preventing excitotoxicity and maintaining synaptic homeostasis. The dysfunction of EAAT5 has been implicated in various neurological disorders, including retinal diseases and neurodegenerative conditions, where impaired glutamate clearance leads to neuronal damage. Given its importance in neurotransmission and neuroprotection, researchers have focused on the characterization and functional analysis of EAAT5, particularly through the generation of recombinant proteins. These recombinant proteins allow for the investigation of EAAT5's structural and functional properties, facilitating studies on its substrate specificity, ion dependence, and regulatory mechanisms. Understanding the pharmacological properties of EAAT5 may lead to the development of targeted therapies for diseases associated with glutamate dysregulation. Furthermore, EAAT5 serves as a valuable model for studying the transport mechanisms of excitatory amino acids, providing insights into the broader transport systems within the central nervous system. As research progresses, the application of recombinant EAAT5 proteins in drug testing and potential therapeutic interventions underscores the relevance of this transporter in both basic neuroscience and clinical contexts.
