Analytical Data
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Gene name
IL-18/IL-1F4
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简介
IL-18/IL-1F4 protein is a pro-inflammatory cytokine that is critical in epithelial barrier repair and coordinates immune responses involving Th1 cells and NK cells. IL-18 binds to IL18R1 and IL18RAP to form a ternary complex that activates NF-κ-B and triggers the synthesis of inflammatory mediators. IL-18/IL-1F4 Protein, Porcine is the recombinant Porcine-derived IL-18/IL-1F4 protein, expressed by E. coli , with tag free.
- Application
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Biological Activity
Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is 36.31 ng/mL in the presence of 15 pg/mL TNF-alpha, corresponding to a specific activity is 2.754×104 U/mg. Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is 36.31 ng/mL in the presence of 15 pg/mL TNF-alpha, corresponding to a specific activity is 2.754×104 U/mg.
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Alternative Names
Interleukin-18; IL-18; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; IGIF; Interleukin 18
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Species
Porcine
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
O19073
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Expression Region
Y36-N192
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Protein Length
Full Length of Mature Protein
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Molecular Weight
18 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Interleukin-18 (IL-18), also known as Interleukin-1 family member 4 (IL-1F4), is a critical pro-inflammatory cytokine involved in the immune response. Initially discovered for its ability to enhance the production of interferon-gamma (IFN-γ) from T cells and natural killer (NK) cells, IL-18 plays a pivotal role in promoting Th1-type immune responses. Its dysregulation has been implicated in various autoimmune diseases, chronic inflammatory conditions, and even cancer, making it a compelling target for therapeutic intervention. The recombinant protein form of IL-18/IL-1F4 allows researchers to study its biological functions and signaling pathways in vitro and in vivo. By producing this cytokine in a recombinant system, scientists can explore its role in immune cell activation, inflammation, and the interplay with other cytokines in the immune network. Furthermore, understanding the mechanisms by which IL-18 contributes to disease pathogenesis can lead to novel treatment strategies, potentially blocking or modulating its pathways for therapeutic purposes. The ongoing research into IL-18/IL-1F4 recombinant proteins continues to offer valuable insights into immune regulation and may pave the way for innovative therapies targeting inflammatory disorders and malignancies. As the field progresses, the unique properties and actions of this cytokine will likely yield new approaches to manage diseases characterized by excessive inflammation and immune dysregulation.
