Analytical Data
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Gene name
Heparinase I 蛋白, B
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简介
Heparinase I protein is an enzyme that degrades heparin and heparan sulfate and can modify extracellular matrix components. In addition to degradation, it also releases growth factors that bind to heparin, affecting the extracellular environment. Heparinase I Protein, B. thetaiotaomicron (His) is the recombinant Heparinase I protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Heparin lyase I family protein
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Species
Others
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
WP_011109372.1
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Expression Region
M1-R376
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AA Sequence
MLTAQTKNTQTLMPLTERVNVQADSARINQIIDGCWVAVGTNKPHAIQRDFTNLFDGKPSYRFELKTEDNTLEGYAKGETKGRAEFSYCYATSDDFRGLPADVYQKAQITKTVYHHGKGACPQGSSRDYEFSVYIPSSLDSNVSTIFAQWHGMPDRTLVQTPQGEVKKLTVDEFVELEKTTFFKKNVGHEKVARLDKQGNPVKDKNGKPVYKAGKPNGWLVEQGGYPPLAFGFSGGLFYIKANSDRKWLTDKDDRCNANPGKTPVMKPLTSEYKASTIAYKLPFADFPKDCWITFRVHIDWTVYGKEAETIVKPGMLDVRMDYQEQGKKVSKHIVDNEKILIGRNDEDGYYFKFGIYRVGDSTVPVCYNLAGYSER
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Protein Length
Full Length
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Molecular Weight
45 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Heparinase I is an enzyme that plays a crucial role in the degradation of heparin and heparan sulfate, glycosaminoglycans involved in various biological processes, including cell signaling, growth factor regulation, and anticoagulation. This enzyme is produced by certain bacteria, such as *Flavobacterium heparinum*, and has garnered significant interest in medicinal and biochemical research due to its ability to modulate the interactions between cells and the extracellular matrix. The recombinant form of Heparinase I, produced through genetic engineering techniques, allows for controlled studies of its enzymatic properties and potential therapeutic applications. Recent research has focused on its potential uses in drug delivery systems, cancer therapy, and as an anticoagulant agent. By elucidating the structure-function relationship of this protein, scientists aim to develop novel strategies for treating diseases where heparin metabolism is disrupted. Furthermore, investigations into its inhibitory mechanisms could lead to the development of inhibitors that selectively target heparan sulfate interactions, opening avenues for targeted therapies in tissue engineering and regenerative medicine. The understanding of Heparinase I as a recombinant protein offers insights not only into its biochemical functioning but also into the therapeutic potential of manipulating glycosaminoglycan interactions in various pathological conditions.
