Analytical Data
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Gene name
NOTCH1
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简介
NOTCH1 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and is critical for cell fate decisions. Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes in the cleavage site enhancer. NOTCH1 Protein, Mouse (CHO, mFc) is the recombinant mouse-derived NOTCH1 protein, expressed by CHO , with C-mFc labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Mouse Notch-1 at 2 μg/mL (100 μL/mL) can bind Rat Jagged 1 with an apparent KD < 2 nM. Measured by its binding ability in a functional ELISA. Immobilized Mouse Notch-1 at 2 μg/mL (100 μL/mL) can bind Rat Jagged 1 with an apparent KD is 1.656 nM.
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Alternative Names
Neurogenic locus notch homolog protein 1; Notch1; Notch 1; Motch A; mT14; p300; Motch
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Species
Mouse
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Source
CHO
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Tag
C-mFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
AAM28905
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Expression Region
A19-Q526
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Protein Length
Partial
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Molecular Weight
100 kDa band in SDS-PAGE under reducing conditions.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NOTCH1 is a critical component of the Notch signaling pathway, which plays a vital role in numerous biological processes, including cell differentiation, proliferation, and apoptosis. Dysregulation of NOTCH1 signaling has been implicated in various diseases, particularly in cancers such as T-cell acute lymphoblastic leukemia (T-ALL) and solid tumors. Research has shown that mutations and chromosomal rearrangements involving NOTCH1 contribute to tumorigenesis, making it a compelling target for therapeutic intervention. In this context, the study of recombinant NOTCH1 proteins is significant for understanding the functional mechanisms of NOTCH1 in both normal and pathological states. By producing and purifying NOTCH1 as a recombinant protein, researchers can dissect its activity, identify binding partners, and explore its interactions within the signaling pathway. This approach enables the development of targeted therapies that can potentially inhibit aberrant NOTCH1 signaling in cancer cells. Furthermore, studying NOTCH1 in a recombinant form allows for the investigation of its structural properties and the consequences of specific mutations, ultimately providing insights into the design of novel pharmacological agents. As the understanding of NOTCH1's role in various diseases continues to evolve, recombinant proteins will remain a crucial tool in biomedical research, facilitating the translation of basic science into innovative therapeutic strategies.
