Analytical Data
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基因名
E-Selectin/CD62E
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简介
E-selectin/CD62E Protein, a cell-surface glycoprotein, crucially mediates immunoadhesion by interacting with SELPLG/PSGL1 for the adhesion of neutrophils to cytokine-activated endothelium. It also potentially influences capillary morphogenesis and interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope, independently of SELPLG sulfation. E-Selectin/CD62E Protein, Human (HEK293, His-Fc) is the recombinant human-derived E-Selectin/CD62E protein, expressed by HEK293 , with C-hFc, C-His labeled tag.
- Application
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别名
E-selectin; ELAM-1; LECAM2; CD62E; SELE
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种属
Human
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表达系统
HEK293
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标签
C-hFc;C-His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P16581
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表达区间
W22-P556
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氨基酸序列
WSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYWIGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKDVGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSGLKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMETMQCMSSGEWSAPIPACNVVECDAVTNPANGFVECFQNPGSFPWNTTCTFDCEEGFELMGAQSLQCTSSGNWDNEKPTCKAVTCRAVRQPQNGSVRCSHSPAGEFTFKSSCNFTCEEGFMLQGPAQVECTTQGQWTQQIPVCEAFQCTALSNPERGYMNCLPSASGSFRYGSSCEFSCEQGFVLKGSKRLQCGPTGEWDNEKPTCEAVRCDAVHQPPKGLVRCAHSPIGEFTYKSSCAFSCEEGFELHGSTQLECTSQGQWTEEVPSCQVVKCSSLAVPGKINMSCSGEPVFGTVCKFACPEGWTLNGSAARTCGATGHWSGLLPTCEAPTESNIP
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蛋白长度
Extracellular Domain
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分子量
140-150 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
E-Selectin, also known as CD62E, is a cell adhesion molecule predominantly expressed on activated endothelial cells. It plays a crucial role in the inflammatory response by mediating the tethering and rolling of leukocytes on the endothelium during the early stages of immune response. E-Selectin binds to specific carbohydrate ligands on leukocytes, facilitating their migration from the bloodstream to sites of inflammation or injury. Given its significant role in various pathological conditions, including atherosclerosis, chronic inflammatory diseases, and cancer metastasis, E-Selectin has become a target for therapeutic interventions. The study of E-Selectin at the molecular level, particularly through the development of recombinant protein forms, allows for a better understanding of its structure-function relationships and interaction dynamics with ligands. This research can pave the way for novel anti-inflammatory therapies and drug delivery systems that can selectively target activated endothelial cells, thereby enhancing treatment efficacy while minimizing side effects. The generation and characterization of E-Selectin/CD62E recombinant proteins enable detailed investigations into its role in immune responses, and potential applications in diagnostic and therapeutic settings are being explored as researchers aim to translate these findings into clinical applications.
