Analytical Data
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Gene name
Cadherin-18
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简介
Cadherin-18, a calcium-dependent cell adhesion protein and member of the cadherin family, regulates cellular adhesion processes. Cadherin-18 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived Cadherin-18 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Determined by its ability to inhibit the proliferation of U251 cells. The ED50 for this effect is 29.97 ng/mL, corresponding to a specific activity is 3.34×104 units/mg. Determined by its ability to inhibit the proliferation of U251 cells. The ED50 for this effect is 29.97 ng/mL, corresponding to a specific activity is 3.34×104 units/mg.
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Alternative Names
Cadherin-18; Cadherin-14; CDH18; CDH14
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Species
Rhesus Macaque
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
F7FMS7
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Expression Region
T25-S608
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AA Sequence
TAHHSSIKVTRNQTKHIEGETEVHHRPKRGWVWNQFFVLEEHMGPDPQYVGKLHSNSDKGDGSVKYILTGEGAGTIFIIDDTTGDIHSTKSLDREQKTHYVLHAQAIDRRTNKPLEPESEFIIKVQDINDNAPKFTDGPYIVTVPEMSDMGTSVLQVTATDADDPTYGNSARVVYSILQGQPYFSVDPKTGVIRTALHNMDREAREHYSVVIQAKDMAGQVGGLSGSTTVNITLTDVNDNPPRFPQKHYQLYVPESAQVGSAVGKIKANDADTGSNADMTYSIINGDGMGIFSISTDKETREGILSLKKPLNYEKKKSYTLNIEGANTHLDFRFSHLGPFKDATMLKIIVGDVDEPPLFSMPSYVMEVYENAKIGTVVGTVLAQDPDSANSLVRYFINYNAEDDRFFNIDANTGTIKTTKVLDREETPWYNITVTASENDNPDLLSHVTVGIRVLDVNDNPPELAREYDIVVCENSKPGQVIHTISATDKDDFANGPRFNFFLDEHLPVNPNFTLKDNEDNTASILTRRRRFSRTVQDVYYLPIMISDGGIPSLSSSSTLTIRVCACERDGRVRTCHAEAFLSS
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Protein Length
Partial
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Molecular Weight
80-90 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Cadherin-18 is a member of the cadherin superfamily, which comprises a large group of transmembrane proteins crucial for cell adhesion and signaling in various biological processes. The significance of Cadherin-18 has been highlighted in various studies due to its unique expression pattern in the central nervous system and its role in neuronal development, synaptic plasticity, and stability. Research has indicated that Cadherin-18 participates in the formation of adherens junctions, influencing the organization and integrity of neuronal networks. Its potential involvement in neurodevelopmental disorders and neurodegenerative diseases further underscores the need for a deeper understanding of this protein. Thus, generating recombinant Cadherin-18 is essential for elucidating its molecular mechanisms and interactions. The production of this protein in a recombinant form allows for comprehensive biophysical and biochemical analyses, paving the way for the investigation of its functions and the potential therapeutic implications in neurological conditions. By characterizing Cadherin-18 at a molecular level, researchers aim to unravel its contributions to cell adhesion processes and its broader implications in neuronal health and disease.
