Analytical Data
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Gene name
RAB7A
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简介
RAB7A is a dynamic small GTPase that coordinates endolysosomal trafficking by cycling between a GTP-bound state and a GDP-bound state. In its active state, RAB7A interacts with various effectors, affecting endosomal maturation, microtubule directed migration, and endosome-lysosomal trafficking. RAB7A Protein, Rat (N-His) is the recombinant rat-derived RAB7A, expressed by E. coli , with N-His labeled tag. ,
- Application
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Alternative Names
Ras-related protein Rab-7a; Ras-related protein BRL-RAS; RAB7A; RAB7
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Species
Rat
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P09527
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Expression Region
M1-C207
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AA Sequence
MTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNERAKASAESCSC
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Protein Length
Full Length
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Molecular Weight
23-25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RAB7A is a small GTPase belonging to the RAS superfamily and plays a crucial role in intracellular vesicular trafficking, particularly in the late endosomal and lysosomal pathways. Its function is vital for processes such as autophagy, nutrient sensing, and the degradation of cellular waste, making it important for maintaining cellular homeostasis. Aberrant RAB7A activity has been linked to various diseases, including neurodegenerative disorders, cancer, and infectious diseases, as it can influence the uptake and transport of pathogens and the clearance of damaged organelles. Researchers have been increasingly focused on studying RAB7A due to its potential as a therapeutic target; understanding its structure and function could lead to novel strategies for treating conditions exacerbated by dysfunctional vesicular transport. Recombinant RAB7A protein studies help elucidate its biochemical properties, signaling pathways, and interactions with effector proteins, thus providing insights into its role in health and disease. The generation of RAB7A recombinant proteins facilitates high-throughput screening and characterizes its interactions, offering foundational knowledge for future drug development aimed at modulating RAB7A's activity in pathological contexts.
