Analytical Data
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Gene name
AP2b1
- Application
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Alternative Names
ADTB2; AP105B; AP2-BETA; CLAPB1; Clathrin assembly protein complex 2 beta large chain; Plasma membrane adaptor HA2/AP2 adaptin beta subunit
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P63010
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Expression Region
Thr2~Phe266
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Molecular Weight
31kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AP2b1, a member of the adaptor protein complex family, plays a crucial role in clathrin-mediated endocytosis, a fundamental cellular process involved in the internalization of membrane receptors and nutrients. Recent studies have highlighted its significance in the regulation of various signaling pathways and its involvement in cellular communication, growth, and differentiation. The proper functioning of AP2b1 is essential for the maintenance of cellular homeostasis and has implications in the pathology of several diseases, including cancer and neurodegenerative disorders. Research focusing on the recombinant expression of AP2b1 protein aims to elucidate its structural and functional roles, providing insights into its mechanisms of action at the molecular level. Recombinant AP2b1 protein can be utilized in biochemical assays, structural biology studies, and potentially as a target for drug development. Understanding the precise interaction mechanisms of AP2b1 with other cellular components could pave the way for therapeutic strategies that manipulate its function, thereby addressing diseases associated with endocytic dysregulation. Overall, the exploration of AP2b1 as a recombinant protein holds significant promise for advancing our knowledge of cellular dynamics and developing novel interventions in related health conditions.
