Analytical Data
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基因名
AGO2/Argonaute-2
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简介
The AGO2/Argonaute-2 protein is critical for RNA-mediated silencing complex (RISC)-mediated gene silencing. In "minimal RISC," AGO2 binds to short guide RNAs, such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), to guide RISC for silencing with complementary mRNAs. AGO2/Argonaute-2 Protein, Mouse (sf9, His, solution) is the recombinant mouse-derived AGO2/Argonaute-2 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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别名
Protein argonaute-2; Argonaute2; mAgo2; eIF-2C 2; Eif2c2
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种属
Mouse
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表达系统
Baculovirus
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8CJG0
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表达区间
M1-A860
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蛋白长度
Full Length
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分子量
105 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Ago2, or Argonaute-2, is a critical protein in the RNA interference (RNAi) pathway, playing a pivotal role in gene regulation and post-transcriptional silencing of messenger RNA (mRNA). As a central component of the RNA-induced silencing complex (RISC), Ago2 binds to small RNA molecules, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs), facilitating their targeting to complementary mRNA sequences. This interaction ultimately leads to mRNA degradation or translational repression, making Ago2 essential for maintaining cellular homeostasis and influencing various biological processes, including development, cell differentiation, and defense against viral infections. Research on Ago2 has gained significant interest due to its implications in cancer biology and therapeutic potential, as aberrations in RNAi pathways are frequently associated with tumorigenesis and disease progression. Moreover, Ago2 is considered a promising target for innovative gene-editing tools and applications in synthetic biology. Understanding the structure, function, and regulatory mechanisms of Ago2 can provide valuable insights into the intricacies of gene expression control and may lead to novel strategies for disease intervention. Thus, the study of Ago2 and its associated pathways remains a dynamic and crucial area of investigation within molecular biology and therapeutic development.
