Analytical Data
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基因名
CD117/c-Kit
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简介
CD117/c-kit is a tyrosine protein kinase receptor for KITLG/SCF that coordinates cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis. Upon KITLG/SCF binding, CD117 activates the pathway and phosphorylates PIK3R1, PLCG1, SH2B2/APS, and CBL. CD117/c-kit Protein, Human (P10721-1, sf9) is the recombinant human-derived CD117/c-kit, expressed by Sf9 insect cells, with GST, Flag labeled tag. The total length of CD117/c-kit Protein, Human (P10721-1, sf9) is 433 a.a..
- Application
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种属
Human
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表达系统
Baculovirus
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P10721-1
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表达区间
T544-V976
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD117, also known as c-Kit, is a receptor tyrosine kinase that plays a crucial role in cell signaling related to cell proliferation, survival, and differentiation. It is predominantly expressed in hematopoietic stem cells, as well as in progenitor and mast cells. Dysregulation of CD117 signaling is implicated in various hematological malignancies, notably in conditions such as chronic myeloid leukemia (CML) and gastrointestinal stromal tumors (GISTs). The discovery of specific mutations in the c-Kit gene, which can lead to enhanced activity of the receptor and resistance to apoptosis, highlighted the importance of targeting CD117 for therapeutic intervention. Recombinant CD117/c-Kit proteins have been developed for both basic research and clinical applications, enabling researchers to dissect the underlying mechanisms of c-Kit-mediated signaling pathways and to identify potential therapeutic targets. Furthermore, these recombinant proteins serve as valuable tools for high-throughput screening of small molecules in drug development aimed at inhibiting the aberrant signaling associated with c-Kit mutations. Overall, the study of CD117/c-Kit recombinant proteins is vital for advancing our understanding of stem cell biology, cancer therapeutics, and innovative treatment strategies for c-Kit-related disorders.
