Analytical Data
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基因名
GPX4
- 应用
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别名
Glutathione peroxidase 4 Short name:GPx-4 Short name:GSHPx-4
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种属
ongo pygmaeus
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q4AEH2
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表达区间
28-197aa(U73S)
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分子量
35 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GPX4, or glutathione peroxidase 4, is a crucial enzyme in the antioxidant defense system of cells. It plays a pivotal role in protecting cells from oxidative stress by reducing lipid peroxides, thereby preventing cellular damage that can lead to various diseases, including cancer and neurodegenerative disorders. The study of GPX4 has gained significant attention due to its involvement in ferroptosis, a form of regulated cell death characterized by iron-dependent lipid peroxidation. Unlike other forms of cell death, ferroptosis is distinctively influenced by the levels of GPX4, making it a potential target for therapeutic interventions. Researchers have been investigating the recombinant expression of GPX4 to better understand its functional mechanisms, potential as a biomarker for disease, and as a therapeutic target. The recombinant protein can be used in various assays to study its enzymatic activity, structural biology, and interactions with other molecular partners. Recent advances in gene editing technologies and protein engineering have facilitated the creation of modified GPX4 variants with enhanced stability or catalytic efficiency, paving the way for novel therapeutic strategies. Understanding the role of GPX4 in cellular protection and death mechanisms ultimately offers promising avenues for drug discovery and therapeutic development in oxidative stress-related diseases.
