Analytical Data
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基因名
PLCb4
- Application
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别名
PI-PLC; Phospholipase C-beta-4; Phosphoinositide phospholipase C-beta-4; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15147
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表达区间
Ala2~Gln250
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分子量
32kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PLCb4 is a member of the phospholipase C (PLC) family, which plays a crucial role in cellular signaling pathways by hydrolyzing phosphatidylinositol 4,5-bisphosphate to generate inositol trisphosphate and diacylglycerol. This process is essential for various physiological functions, including cell growth, differentiation, and apoptosis. Recent studies have highlighted PLCb4's unique expression patterns in the central nervous system and its potential involvement in neurological disorders. Research has also suggested that PLCb4 may interact with specific receptor pathways, influencing neuronal development and synaptic plasticity. Due to its significant role in signaling cascades and the growing interest in neurobiology, the recombinant production of PLCb4 has gained traction for further investigation. The ability to produce and study PLCb4 in a controlled environment allows for a deeper understanding of its enzymatic activity, regulatory mechanisms, and potential as a therapeutic target in diseases such as Alzheimer's and Parkinson's. Understanding the structure-function relationship of PLCb4 through recombinant protein studies could pave the way for novel interventions in treating neurological disorders linked to dysfunctional signaling pathways. Hence, research into PLCb4 not only provides insights into fundamental biological processes but also opens avenues for targeted therapeutic strategies in the realm of neurodegenerative diseases.












