Analytical Data
-
Gene name
PLCb4
- Application
-
Alternative Names
PI-PLC; Phospholipase C-beta-4; Phosphoinositide phospholipase C-beta-4; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-4
-
Species
Human
-
Source
E. coli
-
Tag
N-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q15147
-
Expression Region
Ala2~Gln250
-
Molecular Weight
32kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PLCb4 is a member of the phospholipase C (PLC) family, which plays a crucial role in cellular signaling pathways by hydrolyzing phosphatidylinositol 4,5-bisphosphate to generate inositol trisphosphate and diacylglycerol. This process is essential for various physiological functions, including cell growth, differentiation, and apoptosis. Recent studies have highlighted PLCb4's unique expression patterns in the central nervous system and its potential involvement in neurological disorders. Research has also suggested that PLCb4 may interact with specific receptor pathways, influencing neuronal development and synaptic plasticity. Due to its significant role in signaling cascades and the growing interest in neurobiology, the recombinant production of PLCb4 has gained traction for further investigation. The ability to produce and study PLCb4 in a controlled environment allows for a deeper understanding of its enzymatic activity, regulatory mechanisms, and potential as a therapeutic target in diseases such as Alzheimer's and Parkinson's. Understanding the structure-function relationship of PLCb4 through recombinant protein studies could pave the way for novel interventions in treating neurological disorders linked to dysfunctional signaling pathways. Hence, research into PLCb4 not only provides insights into fundamental biological processes but also opens avenues for targeted therapeutic strategies in the realm of neurodegenerative diseases.
