Analytical Data
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Gene name
HMGCS2
- Application
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Alternative Names
3-hydroxy-3-methylglutaryl coenzyme A synthase
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Species
Mouse
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P54869
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Expression Region
Met1~Tyr167
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Molecular Weight
25kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HMGCS2 (3-hydroxy-3-methylglutaryl-CoA synthase 2) is a crucial enzyme involved in the ketogenic pathway, primarily responsible for generating ketone bodies from fatty acids during periods of fasting or low carbohydrate intake. Its activity is vital for maintaining energy homeostasis, especially in the brain and muscle tissues. Dysregulation of HMGCS2 has been linked to various metabolic disorders, making it a significant target for therapeutic intervention. The recombinant expression of HMGCS2 allows for the study of its structure, function, and regulatory mechanisms in detail. By producing this protein in heterologous systems, researchers can investigate its enzymatic properties, interaction with other metabolic pathways, and the impact of specific mutations associated with metabolic diseases. The characterization of HMGCS2 at the molecular level not only enhances our understanding of its role in metabolism but also provides insights into potential strategies for treating conditions such as obesity, type 2 diabetes, and neurological disorders. Thus, the study of recombinant HMGCS2 is essential for elucidating its biological relevance and for developing novel therapies targeting metabolic imbalances.
