Analytical Data
-
Gene name
COX1
- Application
-
Alternative Names
MT-CO1; MTCO1; COI; COXI; Mitochondrially Encoded Cytochrome C Oxidase I; Cytochrome c oxidase polypeptide I
-
Species
Rat
-
Source
E. coli
-
Tag
Two N- s, His- & SUMO-
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P05503
-
Expression Region
Lys479~Lys514
-
Molecular Weight
20kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
COX1, or cytochrome c oxidase subunit 1, is a critical enzyme in the mitochondrial respiratory chain, playing a pivotal role in cellular energy production through ATP synthesis. It is a key component of complex IV, facilitating the transfer of electrons from cytochrome c to molecular oxygen, which ultimately helps in the formation of water and the production of an electrochemical gradient essential for ATP generation. Research on COX1 recombinant proteins has gained significant attention due to their implications in understanding mitochondrial diseases, aging, and metabolic disorders. Recombinant COX1 allows scientists to study the structure-function relationships of this enzyme, assess the impacts of genetic mutations, and explore the enzyme's interactions with various substrates and inhibitors. Moreover, it serves as a valuable tool in the development of targeted therapies for conditions associated with mitochondrial dysfunction. Additionally, investigations into COX1 may contribute to advancements in synthetic biology and bioengineering, where engineered mitochondrial enzymes can enhance bioenergetic processes in both microbial and mammalian systems. Understanding the dynamics of COX1 through recombinant protein studies enhances our grasp of its biophysical properties, regulatory mechanisms, and roles in various pathological states, thus paving the way for novel therapeutic strategies aimed at combating diseases tied to mitochondrial dysfunction.
