Analytical Data
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Gene name
nrdD
- Application
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Alternative Names
nrdD; b4238; JW4197; Anaerobic ribonucleoside-triphosphate reductase; EC 1.1.98.6; Class III ribonucleoside-triphosphate reductase
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Species
Escherichia coli
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P28903
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Expression Region
1-712aa
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Molecular Weight
84 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NrdD is a crucial enzyme involved in the ribonucleotide reductase (RNR) pathway, which is essential for DNA synthesis and repair in all living organisms. Ribonucleotide reductases convert ribonucleotides into deoxyribonucleotides, the building blocks of DNA, thus playing a vital role in cellular proliferation and growth. The importance of NrdD and its homologs has garnered increasing attention in recent years due to their potential implications in cancer biology and antibiotic resistance. Understanding the structure and function of NrdD can provide insights into the mechanisms of DNA metabolism and repair, which are fundamental processes in cellular biology. Research on NrdD recombinant proteins has become a focal point in molecular biology, as these proteins can be engineered for therapeutic applications. Recombinant NrdD can serve as a valuable tool in studying the biochemical pathways it regulates, as well as in high-throughput screening for new inhibitors that could serve as potential drugs. Furthermore, the biochemical properties of NrdD make it an interesting target for further studies on enzyme kinetics and regulation, providing opportunities for advances in biochemistry and biotechnology. Overall, the investigation of NrdD recombinant proteins is a promising area that bridges fundamental research with practical applications, seeking to unravel the complexities of DNA synthesis and its implications in health and disease.
