Analytical Data
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基因名
NFKBIE/IKB epsilon
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简介
NFKBIE/IKB epsilon Protein crucially inhibits NF-kappa-B by forming a cytoplasmic complex, preventing its DNA-binding activity. It targets specific complexes involving p50-p65, p50-c-Rel, and interacts with subunits RELA, REL, NFKB1 (p50), and NFKB2 (p52). NFKBIE/IKB epsilon plays a pivotal role in regulating NF-kappa-B signaling, controlling nuclear translocation and transcriptional activities. NFKBIE/IKB epsilon Protein, Human (His) is the recombinant human-derived NFKBIE/IKB epsilon protein, expressed by E. coli , with N-10*His labeled tag.
- Application
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别名
C20orf18; HOIL-1; HOIL1; PBMEI; PGBM1; RBCK2; RNF54; UBCE7IP3; XAP3; XAP4; ZRANB4
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种属
Human
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表达系统
E. coli
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标签
N-10*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
O00221
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表达区间
M1-D500
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氨基酸序列
MNQRRSESRPGNHRLQAYAEPGKGDSGGAGPLSGSARRGRGGGGAIRVRRPCWSGGAGRGGGPAWAVRLPTVTAGWTWPALRTLSSLRAGPSEPHSPGRRPPRAGRPLCQADPQPGKAARRSLEPDPAQTGPRPARAAGMSEARKGPDEAEESQYDSGIESLRSLRSLPESTSAPASGPSDGSPQPCTHPPGPVKEPQEKEDADGERADSTYGSSSLTYTLSLLGGPEAEDPAPRLPLPHVGALSPQQLEALTYISEDGDTLVHLAVIHEAPAVLLCCLALLPQEVLDIQNNLYQTALHLAVHLDQPGAVRALVLKGASRALQDRHGDTALHVACQRQHLACARCLLEGRPEPGRGTSHSLDLQLQNWQGLACLHIATLQKNQPLMELLLRNGADIDVQEGTSGKTALHLAVETQERGLVQFLLQAGAQVDARMLNGCTPLHLAAGRGLMGISSTLCKAGADSLLRNVEDETPQDLTEESLVLLPFDDLKISGKLLLCTD
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蛋白长度
Full Length
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分子量
57 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NFKBIE, also known as IκB epsilon, is a crucial regulator of the NF-κB signaling pathway, which plays a significant role in various physiological and pathological processes, including inflammation, immune response, and cell survival. NF-κB proteins are typically sequestered in the cytoplasm by IκB proteins, preventing their translocation to the nucleus. Upon stimulation, IκBs are phosphorylated, leading to their degradation and the subsequent activation of NF-κB. NFKBIE is unique among the IκB family due to its specific role in modulating NF-κB's transcriptional activity in response to diverse stimuli, such as cytokines and stress signals. Research into NFKBIE includes its role in diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. Understanding the function and regulation of NFKBIE has implications for developing targeted therapies that can modulate NF-κB activity. Recombination technology is often employed to produce NFKBIE protein for biochemical studies, enabling researchers to investigate its structure, function, and interactions with other molecules. This research is critical for elucidating the complex mechanisms of NF-κB signaling and its impact on human health and disease.
