Analytical Data
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基因名
Semaphorin-5A/SEMA5A
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简介
Semaphorin-5A (SEMA5A) protein lacks essential conserved residue(s) for propagating feature annotation. Semaphorin-5A/SEMA5A Protein, Mouse (HEK293, His) is the recombinant mouse-derived Semaphorin-5A/SEMA5A protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by the ability of the immobilized protein to support the adhesion of PANC-1 human pancreatic cancer cells. The ED50 for this effect is 1.226 μg/mL, corresponding to a specific activity is 815.661 units/mg. Measured by the ability of the immobilized protein to support the adhesion of PANC-1 human pancreatic cancer cells. The ED50 for this effect is 1.226 μg/mL, corresponding to a specific activity is 815.661 units/mg.
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别名
Semaphorin-5A; Semaphorin-F; Sema F; SEMA5A; SEMAF
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种属
Mouse
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q3UPZ0
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表达区间
E23-T765
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氨基酸序列
ETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKELVVGARNYLFRLELEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQGTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPNPNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHLAVDVVQGRETLVHIIYLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERRSEPIRSLQILHSQSVLFVGLQEHVAKIPLKRCHFHQTRSACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSIPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAVGSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNACPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCST
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蛋白长度
Partial
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分子量
95-110 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Semaphorin-5A (SEMA5A) is a member of the semaphorin family, which plays crucial roles in neuronal development and guidance. Initially identified for its function in cellular signaling during neural development, SEMA5A is known to interact with specific receptors, influencing processes such as axon guidance, synapse formation, and cell migration. Recent studies have expanded its importance beyond the nervous system, suggesting its involvement in various physiological and pathological processes, including immune response, cardiovascular development, and cancer progression. The investigation of recombinant SEMA5A proteins has gained traction as researchers aim to characterize its structural properties and functional roles in both normal and disease states. The generation of these recombinant proteins allows for in-depth studies on SEMA5A's mechanisms of action, receptor interactions, and potential therapeutic applications. Moreover, understanding the signaling pathways regulated by SEMA5A can facilitate the development of novel treatments targeting neurodevelopmental disorders, immune diseases, and tumor progression. Overall, the study of recombinant SEMA5A proteins holds promise for elucidating its diverse biological functions and therapeutic potential.
