Analytical Data
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基因名
Galectin-1/LGALS1
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简介
Galectin-1/LGALS1 protein is a β-galactoside-binding lectin that regulates apoptosis, cell proliferation and differentiation by inhibiting CD45 protein phosphatase activity and preventing Lyn kinase dephosphorylation. As a homodimer, it induces T cell apoptosis and interacts with CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, CD45, laminin, and SUSD2. Galectin-1/LGALS1 Protein, Rat is the recombinant rat-derived Galectin-1/LGALS1 protein, expressed by E. coli , with tag free.
- Application
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生物活性
Measured in a cell proliferation assay using HUVEC cells. The ED50 for this effect is 0.4036 μg/mL, corresponding to a specific activity is 2.477×10^3 units/mg. Measured in a cell proliferation assay using HUVEC cells. The ED50 for this effect is 0.4036 μg/mL, corresponding to a specific activity is 2.477×10^3 units/mg.
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别名
Galectin-1; Gal-1; HLBP14; Galaptin; HBL; LGALS1
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种属
Rat
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P11762
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表达区间
A2-E135
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蛋白长度
Full Length of Mature Protein
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分子量
13 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Galectin-1, also known as LGALS1, is a member of the galectin family of carbohydrate-binding proteins, which play crucial roles in various biological processes, including cell-cell interactions, immune response regulation, and apoptosis. It is primarily expressed in a variety of tissues, and its aberrant expression has been implicated in several pathologies, including cancer, autoimmune diseases, and fibrosis. The protein functions by recognizing and binding to specific glycoconjugates on the surface of cells, thereby influencing cellular signaling pathways and promoting tumor progression through mechanisms such as immune evasion and enhanced tumor cell survival. Due to its significant involvement in disease processes, Galectin-1 has attracted considerable research interest as a potential therapeutic target. The development of recombinant Galectin-1 proteins has facilitated a deeper understanding of its structural characteristics, binding properties, and functional roles in biological systems. These studies not only enhance our knowledge of galectin biology but also pave the way for novel therapeutic strategies that could exploit the modulation of Galectin-1 activity to treat various diseases, notably in oncology and immunology. Furthermore, the recombinant form allows for the exploration of its potential as a biomarker for disease progression and treatment response, thereby supporting the development of personalized medicine approaches. Overall, research on Galectin-1/LGALS1 recombinant proteins is integral to elucidating its dual role as a mediator of both protective and pathological processes in human health and disease.
