Analytical Data
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Gene name
Galectin-1/LGALS1
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简介
Galectin-1/LGALS1 protein is a β-galactoside-binding lectin that regulates apoptosis, cell proliferation and differentiation by inhibiting CD45 protein phosphatase activity and preventing Lyn kinase dephosphorylation. As a homodimer, it induces T cell apoptosis and interacts with CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, CD45, laminin, and SUSD2. Galectin-1/LGALS1 Protein, Rat is the recombinant rat-derived Galectin-1/LGALS1 protein, expressed by E. coli , with tag free.
- Application
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Biological Activity
Measured in a cell proliferation assay using HUVEC cells. The ED50 for this effect is 0.4036 μg/mL, corresponding to a specific activity is 2.477×10^3 units/mg. Measured in a cell proliferation assay using HUVEC cells. The ED50 for this effect is 0.4036 μg/mL, corresponding to a specific activity is 2.477×10^3 units/mg.
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Alternative Names
Galectin-1; Gal-1; HLBP14; Galaptin; HBL; LGALS1
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Species
Rat
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P11762
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Expression Region
A2-E135
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Protein Length
Full Length of Mature Protein
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Molecular Weight
13 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Galectin-1, also known as LGALS1, is a member of the galectin family of carbohydrate-binding proteins, which play crucial roles in various biological processes, including cell-cell interactions, immune response regulation, and apoptosis. It is primarily expressed in a variety of tissues, and its aberrant expression has been implicated in several pathologies, including cancer, autoimmune diseases, and fibrosis. The protein functions by recognizing and binding to specific glycoconjugates on the surface of cells, thereby influencing cellular signaling pathways and promoting tumor progression through mechanisms such as immune evasion and enhanced tumor cell survival. Due to its significant involvement in disease processes, Galectin-1 has attracted considerable research interest as a potential therapeutic target. The development of recombinant Galectin-1 proteins has facilitated a deeper understanding of its structural characteristics, binding properties, and functional roles in biological systems. These studies not only enhance our knowledge of galectin biology but also pave the way for novel therapeutic strategies that could exploit the modulation of Galectin-1 activity to treat various diseases, notably in oncology and immunology. Furthermore, the recombinant form allows for the exploration of its potential as a biomarker for disease progression and treatment response, thereby supporting the development of personalized medicine approaches. Overall, research on Galectin-1/LGALS1 recombinant proteins is integral to elucidating its dual role as a mediator of both protective and pathological processes in human health and disease.
