Analytical Data
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Gene name
GJb1
- Application
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Alternative Names
CMTX1; CX32; GJ-B1; Connexin 32; Charcot-Marie-Tooth Neuropathy,X-Linked; GAP junction 28 kDa liver protein
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Species
Human
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Source
E. coli
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Tag
N- His & GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P08034
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Expression Region
Met1~Thr130
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Molecular Weight
44kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GJB1, also known as connexin 32, is a gene that encodes a protein important for the formation of gap junctions, which facilitate intercellular communication. Mutations in the GJB1 gene are associated with X-linked Charcot-Marie-Tooth disease (CMTX), a hereditary neuropathy characterized by progressive muscle weakness and atrophy, sensory loss, and neuropathic pain. The study of GJB1 recombinant protein aims to better understand the structure-function relationship of connexin proteins and their role in nerve cell communication. Investigations into the GJB1 protein can provide insights into the pathological mechanisms underlying CMTX and similar neurodegenerative disorders. Moreover, recombinant expression systems are employed to produce GJB1 protein for biochemical assays and structural studies, helping to elucidate how mutations disrupt its functions. This research not only contributes to the understanding of genetic factors in peripheral neuropathies but may also pave the way for the development of targeted therapies to mitigate symptoms or slow the progression of diseases linked to connexin dysfunction. As the field of protein studies evolves, GJB1 serves as a vital model for exploring potential interventions in connexin-related pathological conditions.
