Analytical Data
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基因名
RecF/UvrF
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简介
RecF/UvrF Protein is crucial in DNA metabolism, essential for DNA replication and inducing the SOS response. Its preference for binding to single-stranded DNA underscores its role in DNA maintenance. RecF also displays an affinity for ATP binding, emphasizing its involvement in regulating DNA-related functions. RecF/UvrF Protein, E.coli (His-SUMO) is the recombinant E. coli-derived RecF/UvrF protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.
- Application
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别名
recF; uvrF; b3700; JW3677; DNA replication and repair protein RecF
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种属
E.coli
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表达系统
E. coli
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标签
N-SUMO;N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0A7H0
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表达区间
S2-D357
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氨基酸序列
SLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD
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蛋白长度
Full Length of Mature Protein
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分子量
56.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The RecF/UvrF protein system plays a crucial role in the DNA repair and recombination processes in bacteria, notably in Escherichia coli. This system is essential for maintaining genomic stability, particularly under conditions of DNA damage caused by environmental factors or replication errors. RecF, a key component of this pathway, is involved in single-stranded DNA binding and is critical for the recruitment of other recombination proteins to the site of damage. UvrF, on the other hand, is part of the UvrABC endonuclease complex responsible for nucleotide excision repair, which removes bulky DNA adducts. Research on the functional interactions between RecF and UvrF has revealed insights into the mechanisms of homologous recombination and the coordinated response to DNA lesions, shedding light on the cellular reactions to genotoxic stress. Understanding the molecular dynamics of this system not only clarifies bacterial DNA repair strategies but also has implications for developing new antibacterial agents and improving our knowledge of evolutionary processes and horizontal gene transfer. As bacterial resistance to antibiotics remains a significant challenge, insights into the RecF/UvrF system can potentially guide the design of novel therapeutic strategies aimed at disrupting DNA repair pathways in pathogenic bacteria.
