Analytical Data
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Gene name
rpsG
- Application
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Alternative Names
rpsG; b3341; JW3303; 30S ribosomal protein S7; Small ribosomal subunit protein uS7
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Species
Escherichia coli
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P02359
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Expression Region
2-179aa
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Molecular Weight
23.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of the rpsG gene, which encodes the ribosomal protein S7, is pivotal in understanding the intricacies of protein synthesis within prokaryotic cells. Ribosomal proteins play critical roles in the structure and function of ribosomes, the cellular machinery responsible for translating genetic information into proteins. Abnormalities or mutations in rpsG can lead to defects in ribosome assembly, impacting the overall cellular metabolism and growth. Research on recombinant rpsG protein involves cloning the gene into expression vectors, producing the protein in host systems such as E. coli, and purifying it for further analysis. These studies not only provide insights into the functional mechanisms of ribosomes but also explore potential implications in antibiotic resistance, as certain antibiotics target ribosomal function. Furthermore, understanding the properties of rpsG and its interactions with ribosomal RNA and other proteins can reveal novel aspects of ribosome biogenesis and dynamics. This research ultimately contributes to a broader comprehension of cellular biology and has potential applications in developing therapeutic strategies to combat bacterial infections.
