Analytical Data
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Gene name
rpsH
- Application
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Alternative Names
rpsH; b3306; JW3268; 30S ribosomal protein S8; Small ribosomal subunit protein uS8
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Species
Escherichia coli
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A7W7
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Expression Region
2-130aa
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Molecular Weight
18 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RpsH is a highly conserved protein that plays a crucial role in the assembly and function of the ribosome in bacteria, particularly in the structure and stability of the ribosomal RNA. Its primary function is to bind to the 30S ribosomal subunit, facilitating the proper folding of rRNA and the assembly of ribosomal proteins, which are essential for protein synthesis. Recent studies have highlighted RpsH's importance not just in ribosome function but also in the stringent response, a mechanism that bacteria use to adapt to nutritional stress by modulating their growth and metabolic activities. Mutations or deletions in the rpsH gene can lead to impaired ribosomal assembly, resulting in growth defects and altered resistance to antibiotics. Due to its fundamental role in bacterial physiology, RpsH has garnered attention as a potential target for novel antimicrobial therapies. Researchers are now focusing on producing recombinant RpsH protein to study its structural properties, interaction with rRNA, and its role in ribosome assembly in greater detail. By understanding the intricacies of RpsH function, scientists aim to explore new avenues for drug development that can combat antibiotic resistance and improve our comprehension of bacterial ribosome biogenesis.
