Analytical Data
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基因名
ANAPC5
- Application
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别名
Cyclosome subunit 5
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种属
Human
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表达系统
E. coli
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标签
N- GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UJX4
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表达区间
2-232aa
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分子量
53.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ANAPC5, a vital component of the Anaphase Promoting Complex/Cyclosome (APC/C), plays a crucial role in cell cycle regulation, particularly in the transition from metaphase to anaphase during mitosis. The APC/C is a multi-subunit E3 ubiquitin ligase that targets specific proteins for ubiquitination and subsequent degradation by the proteasome, thus ensuring proper chromosome segregation and maintaining genomic stability. Dysregulation of ANAPC5 and the APC/C can lead to various human diseases, including cancer, where improper cell division and genomic instability are prevalent. Research on the recombinant ANAPC5 protein has gained momentum to better understand its structure, function, and interactions within the APC/C complex. Recombinant protein expression allows for detailed biochemical and structural studies, facilitating insights into the mechanisms through which ANAPC5 influences cell cycle progression. By exploring the precise roles and regulatory mechanisms of ANAPC5, researchers aim to uncover potential therapeutic targets for conditions associated with cell cycle dysregulation. Moreover, studying this protein contributes to our broader understanding of eukaryotic cell biology, emphasizing the importance of proper mitotic regulation in health and disease.
