Analytical Data
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Gene name
Annexin A5/ANXA5
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简介
Annexin A5 Protein, Human is a 35 kDa multifunctional protein which belongs to the annexin family. Annexin A5 induces in vitro fusion and aggregation of vesicles in a Ca2+- and acidic-pH-dependent manner.Annexin A5 is accepted as an early marker of apoptosis. Annexin A5 has anti-thrombotic and anti-microbial properties[1].
- Application
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Biological Activity
Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 0.1537 μg/mL. Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 0.1537 μg/mL. Corresponding to a specific activity is 6506.181 units/mg.
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Alternative Names
rHuAnnexin A5; ANXA5; Annexin A5; Annexin V; Calphobindin I (CPB-I); Endonexin II; Anchorin CII; ANX5; ENX2; PP4
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P08758
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Expression Region
A2-D320
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AA Sequence
AQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQRQEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQRMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMIKGDTSGDYKKALLLLCGEDD
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Protein Length
Full Length of Mature Protein
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Molecular Weight
33.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Annexin A5 (ANXA5) is a member of the annexin family of proteins, which are known for their ability to bind to phospholipids in a calcium-dependent manner. This protein plays a crucial role in various cellular processes, including apoptosis, cell migration, and membrane repair. The study of recombinant ANXA5 has garnered significant interest due to its potential applications in both basic research and clinical settings. In cellular contexts, ANXA5 is often implicated in the prevention of apoptosis and the modulation of inflammatory responses, making it a valuable target for therapeutic interventions in diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions. The generation of recombinant ANXA5 allows researchers to investigate its biochemical properties, functional mechanisms, and interactions with other cellular components in detail. Furthermore, the use of recombinant protein technology has facilitated the production of modified forms of ANXA5, enhancing its properties for use in diagnostics and therapeutics. With ongoing research, ANXA5 continues to emerge as a promising biomarker and target for novel therapeutic strategies, underscoring the need for a deeper understanding of its biological functions and the development of effective applications in medicine.
