Analytical Data
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基因名
PIAS1
- Application
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别名
GBP; DDXBP1; GU/RH-II; ZMIZ3; Gu-binding protein; Zinc Finger,MIZ-Type Containing 3; DEAD/H(Asp-Glu-Ala-Asp/His)Box Binding Protein 1; RNA helicase II-binding protein
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种属
Human
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75925
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表达区间
Glu409~Pro645
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分子量
29kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PIAS1 (Protein Inhibitor of Activated STAT1) is an E3 ubiquitin ligase that plays a crucial role in regulating various cellular processes, including immune responses, gene expression, and signal transduction pathways. It modulates the activity of STAT proteins, which are pivotal in mediating cellular responses to cytokines and growth factors. Given its involvement in key regulatory mechanisms, aberrations in PIAS1 function can contribute to various diseases, including autoimmune disorders and cancer. Recent studies have highlighted the significance of PIAS1 in the regulation of the NF-κB and p53 pathways, further emphasizing its potential as a therapeutic target. The recombinant expression of PIAS1 in various systems facilitates the investigation of its structural characteristics and functional dynamics, providing insights into its ligase activity and interaction with substrate proteins. Through the use of recombinant PIAS1, researchers aim to decipher its role in post-translational modifications and elucidate its impact on cellular signaling networks. This research is critical for advancing our understanding of PIAS1’s contributions to cellular dysfunctions and for developing innovative strategies to manipulate its activity for therapeutic benefit.
