Analytical Data
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Gene name
SGK1
- Application
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Alternative Names
Serine/threonine-protein kinase Sgk1
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Species
Mouse
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9WVC6
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Expression Region
Ser145~Asp386
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Molecular Weight
30kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SGK1 (serum/glucocorticoid-regulated kinase 1) is a serine/threonine kinase that plays a crucial role in various cellular processes, including cell survival, proliferation, and stress response. It is a member of the AGC (PKA, PKG, and PKC) kinase family and is known to be involved in the signaling pathways activated by growth factors and hormones. Since SGK1 is implicated in numerous physiological and pathological conditions, such as cancer, obesity, and cardiovascular diseases, researchers have focused on understanding its molecular mechanisms and functional roles. The recombinant expression of SGK1 protein has gained attention as it enables detailed studies of its biochemical properties, substrate specificity, and interactions with other signaling molecules. By producing SGK1 as a recombinant protein, scientists can investigate its enzymatic activity, identify potential inhibitors, and characterize its structural features using techniques like X-ray crystallography and NMR spectroscopy. Furthermore, studying SGK1 in a recombinant form allows for the exploration of its therapeutic potential in diseases where its dysregulation is a factor. Overall, SGK1 recombinant protein research is significant for uncovering new insights into cellular signaling mechanisms and developing targeted therapies for diseases associated with aberrant SGK1 activity.
