Recombinant Human DAT Protein，His & GST

Dopamine transporter (DAT) is a critical protein involved in the reuptake of dopamine from the synaptic cleft into presynaptic neurons, playing an essential role in regulating dopamine signaling in the brain. Abnormalities in DAT function are linked to various neurological and psychiatric disorders, including Parkinson's disease, attention deficit hyperactivity disorder (ADHD), and substance use disorders. Researchers have focused on recombinant DAT proteins to better understand their structure, function, and interactions with ligands, including drugs that target dopamine receptors. This research has implications for drug development and the treatment of dopaminergic dysfunctions. Structural studies involving X-ray crystallography and cryo-electron microscopy have provided insights into the conformational dynamics of DAT and its binding sites, shedding light on how different substances modulate its activity. Additionally, the use of recombinant techniques allows for the production of DAT variants to study specific mutations and their effects on transporter function. Overall, the investigation of DAT and its recombinant forms is crucial for elucidating the molecular mechanisms of dopamine transport and developing therapeutic strategies for dopamine-related disorders.