Analytical Data
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Gene name
DDB1
- Application
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Alternative Names
XAP1
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Species
Human
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Source
Baculovirus
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16531-1
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Expression Region
S2-H1140
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Protein Length
Full Length of Isoform-1 Mature Protein
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Molecular Weight
128.9 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DDB1 (Damage-specific DNA-binding protein 1) is a crucial component of the DNA damage recognition and repair pathway, particularly in the context of nucleotide excision repair (NER). It forms a part of the DDB complex, which plays a pivotal role in recognizing and binding to DNA lesions caused by ultraviolet (UV) radiation and other DNA-damaging agents. Research has highlighted that DDB1 not only facilitates the recruitment of repair factors to sites of damage but also serves as a substrate adaptor for cullin-RING E3 ubiquitin ligases, thereby influencing protein degradation and cellular responses to DNA damage. Misregulation of DDB1 has been implicated in various cancers, making it a potential target for therapeutic interventions. Furthermore, the study of recombinant DDB1 protein is essential for understanding its structural and functional dynamics in DNA repair mechanisms. Characterizing DDB1 through recombinant expression systems allows for in-depth investigations into its interaction with DNA and other protein partners, elucidating its role in maintaining genomic stability. Overall, the research on DDB1 and its recombinant forms contributes significantly to our understanding of the molecular basis of DNA repair processes and their implications in disease.
